Generator
Part:BBa_K4242010:Design
Designed by: zongheng Wei Group: iGEM22_CUG-China (2022-09-29)
Pci+RBS+fleQ+TT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 943
Illegal XhoI site found at 1396 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 336
Illegal NgoMIV site found at 1344
Illegal NgoMIV site found at 1356
Illegal NgoMIV site found at 1432
Illegal NgoMIV site found at 1517
Illegal AgeI site found at 745 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 358
Illegal SapI.rc site found at 1291
Illegal SapI.rc site found at 1300
Design Notes
This is a composite part that could produce FleQ in the plasmid.
Source
Use RBS to produce FleQ protein under the promoter Pci regulates and use the GFP protein to reflect FleQ working under the control of Pci in the plasmid.